Inexpensive cartridge for the collection of radioactive methyl esters from gas-liquid chromatographs.
نویسندگان
چکیده
Glass wool was substituted for anthracene in glass cartridges designed for the collection of methylated fatty acids in the effluent stream from a gas-liquid chromatograph. Inexpensive cartridges that gave the same results as those filled with anthracene were obtained.
منابع مشابه
Determination of the specific radioactivity of fatty acids separated as their methyl esters by gas-liquid chromatography.
Free or combined (3)H-labeled fatty acids are converted to their methyl-(14)C esters or, if labeled with (14)C, to their methyl-(3)H esters. For a given specific radioactivity of the methyl group, the nuclide ratio in the esters separated by GLC is a direct measure of the specific radioactivity of the fatty acids, and quantitative collection is unnecessary. Methods of methylation with minimum q...
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Fatty acid methyl esters are the major radioactive lipid products obtained after incubation of rat lung membranes with [methyl-3H or 14C]S-adenosylmethionine. Evidence which suggests an enzymatic transmethylation includes: time and protein dependence, lack of reaction at 0 degrees C or with heat-denatured membranes, an apparent affinity for S-adenosylmethionine of about 1 microM, inhibition by ...
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A novel method is presented for transesterification of fatty acid esters in phospholipids and triglycerides to benzyl esters while simultaneously recovering free fatty acids as methyl esters. Transesterification is catalyzed by 0.2 M (m-trifluoromethyl phenyl)trimethyl ammonium hydroxide in methylene chloride, 10% (v/v) benzyl alcohol, and 1% (w/v) potassium tert-butoxide, and is complete in 30...
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Migration of chemicals from plastic containers into drinks and liquids containing them, is supposed to be a hazardous phenomenon and results in many health problems. Sample preparation is of great importance due to trace amounts analysis of these compounds. In this research, dispersive liquid–liquid microextraction is applied for the extraction and preconcentration of the migrated compounds pri...
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عنوان ژورنال:
- Journal of lipid research
دوره 7 3 شماره
صفحات -
تاریخ انتشار 1966